檔案名稱：huttlrna.htm

人類組織RNA

Human Total RNA

利用電子郵件訂購生工公司人類組織RNA

Product Description:

MDBio's Human Total RNA is isolated from quick-frozen tissue to ensure the highest quality products. Denaturing agarose gel electrophoresis and Northern Blotting are performed to ensure the integrity of mRNA. Total RNA is supplied in ethanol precipitation or water free of RNase. Those items should stored in deep freezer and specialized for research use only.

產品
代碼		 組織 包裝
規格		 價格 交貨
時間
22001 Brain 100ug 4200元 兩週
22002 Heart 100ug 4200元 兩週
22003 Stomach 100ug 4200元 兩週
22004 Kidney 100ug 4200元 兩週
22005 Liver 100ug 4200元 兩週
22006 Lung 100ug 4200元 兩週
22007 Muscle 100ug 4200元 兩週
22008 Small Intestine 100ug 4200元 兩週
22009 Colon 100ug 4200元 兩週
22010 Spleen 100ug 4200元 兩週
22011 Skin 100ug 4200元 兩週
22012 Testis 100ug 4200元 兩週
22013 Duodenun 100ug 4200元 兩週
22014 Rectum 100ug 4200元 兩週
22015 Esophagus 100ug 4200元 兩週
22016 Pancreas 100ug 4200元 兩週
22017 Thymus 100ug 4200元 兩週
22018 Salivary Gland 100ug 4200元 兩週
22019 Prostate 100ug 4200元 兩週
22020 Bladder 100ug 4200元 兩週
22021 Lymph Node 100ug 4200元 兩週
22022 Uterus 100ug 4200元 兩週
22023 Ovary 100ug 4200元 兩週
22024 Fetus Liver 100ug 4200元 兩週
22025 Fetus Kidney 100ug 4200元 兩週
22026 Fetus Brain 100ug 4200元 兩週
22027 Fetus Lungs 100ug 4200元 兩週
22028 Bone Marrow 100ug 4200元 兩週
22029 Spinal Cord 100ug 4200元 兩週
22030 Thyroid 100ug 4200元 兩週
22031 Placenta 100ug 4200元 兩週
22032 Mammary Gland 100ug 4200元 兩週

Introduction:

1. MDBio's Human Total RNA is supplied legitimately, and we will not undertake any responsibility beyond the products.
2. The studies based on our Human Total RNA should be done upon morality and principles, and should not against the law.
3. MDBio's Total RNA listed is isolated from 3 to 5 cases of quick-frozen tissue.
4. Besides the adults total RNA, we can also supply Total RNA isolated from some fetal tissues.
5. All Total RNA supplied is quantified, metamorphic denaturing electrophoresis analyzed and Northern Blot analyzed.

Metamorphic Denaturing Electrophoresis Analysis:

Total RNA is heated at 70℃ for 30 minutes, then the integrity of 18S and 28S ribosomal RNA are checked by denaturing agarose gel electrophoresis. No difference between heated and unheated samples should be observed (Fig. 1).

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Fig. 1 - Denaturing Agarose Gel Electrophoresis of 70℃ Heated and Unheated Total RNA Sample.

Northern Blot Analysis:

Each lot of RNA is Northern blotted and probed with actin and fibronectin to ensure the integrity of high molecular weight mRNA.

Loading of the total RNA from each tissue was adjusted to reflect approximately equal amounts of actin mRNA. The fluctuation of beta-actin signals between different lanes (except that of skeletal muscle and heart, whose signals are set to be twice as the average of other lanes) is set to be less than 60%.

Each lane probed with actin and fibronectin to ensure the integrity of high molecular weitght mRNA. For actin hybridization, the signal to noise ratio is set to be not less than 5. For fibronectin hybridization, the signal to noise ratio is set to be not less than 2.

Fig. 2 - Phosphor image of Hu-MTNB-01 Hybridized with Radioactive Beta-actin Probe

採購找生工，預算最輕鬆。

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中華民國95年07月17日更新